![]() Samples with a diverse or unknown bacterial composition-such as for metagenomic-are more complex. 3/5 M Potassium acetate, pH 6 (Neutralization buffer).Using concentrated potassium acetate causes proteins and chromosomal DNA to precipitate while the plasmid DNA remains in solution and can be isolated using a silica spin column. Plasmid-containing bacteria are lysed with a SDS/NaOH solution. ![]() coli using an alkaline lysis is a well-established method.
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